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Open up NEBCloner and select digestion. Next, choose the two enzymes that you would like to digest simultaneously. Please note that the second enzyme is optional. The tool will give you a protocol with just one enzyme as well.

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We recommend using the Double Digest Finder and use the buffer it recommends. For example, you have old buffer NEBuffer 1 with one of the enzymes and CutSmart Buffer with the second enzyme. If Double Digest Finder recommends to use NEBuffer 1.1, then you can use NEBuffer 1 + BSA for the double digest.

I've done double digests in the past, so I know they can be done effectively, I just needed to know if it was possible with these enzymes. Thanks all for your help, all the best, Shaun. Cite Double Digest Finder.

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Please note that the second enzyme is optional.

Upon running a gel, I noticed that there were two distinct bands in the doulbe-digest (see picture).
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neb.com/tools-and-resources/interactive-tools/double-digest- 23 Jan 2010 New England Biolabs' (NEB) popular web tools, Double Digest Finder and Enzyme Finder, are now available for the iPhone™ and iPod® Digesting a DNA substrate with two restriction endonucleases simultaneously ( double digestion) is a common timesaving procedure.

Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled quality. Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart™ Buffer (over 215 enzymes are available in the same buffer) simplifying double digests 关于NEB.
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Selecting the best Please use NEB Double Digest Finder to perform a double digest calculation. Suggested NEBuffers for Double Digestion. Enzy me. AatII. AvrII. Bam. 9 Jul 2016 The introduction of universal buffers has made double digests easier. Universal buffers, such as the CutSmart buffer from NEB, are convenient is a 10X buffer supplied with over 215 restriction enzymes, simplifying double digests.